Prediction of ace-1 resistance mutation sites in the acetylcholinesterase gene of Spodoptera frugiperda based on high-throughput sequencing big data

Prediction of ace-1 resistance mutation sites in the acetylcholinesterase gene of Spodoptera frugiperda based on high-throughput sequencing big data

Spodoptera frugiperda is a major worldwide migratory agricultural pest, ranking first in the list of crop pests and diseases of China.  It invaded China in 2019 and was first discovered in Liaoning Province in 2020.  A large number of studies have shown that reduced target sensitivity is the main mechanism of the insect pest’s high-level resistance to insecticides. Obtaining target gene mutation sites is essential for the research of insecticide resistance and the guidance of pesticide application for crop production. In this paper, 38 genome high-throughput sequencing data and 43 transcriptome sequencing data from Brazil and China were analyzed to predict the mutation of the acetylcholinesterase gene ace-1 of S. frugiperda that invaded China by constructing a bioinformatics analysis pipeline. The results showed that the acetylcholinesterase gene ace-1 of S. frugiperda that invaded China has two known resistance mutation sites, namely A201S (Ala→Ser) and F290V (Phe→Val), and no G227A (Gly→ Ala) resistance site mutation, however, there is a novel potential different point mutation site of Gly→Ala.